Measurement of Nattokinase Concentration in Blood
Measurement of Nattokinase Concentration in Blood
Japanese Develop Enzyme-Linked Immunosorbent Assay (ELISA)
By Peter A. McCullough, MD, MPH
A doctor asked me at a meeting recently if Nattokinase can be measured in the blood, so I decided to investigate. Many of you know that Nattokinase is a key component of McCullough Protocol BSD (Base Spike Detoxification) to assist in the management of long-COVID and vaccine injury syndromes.
We know that Nattokinase is active after oral absorption because the D-dimer and other measures of hemostasis and thrombolysis change within an hour or two. However, a blood test to measure levels of the drug would be useful.
Tanikawa, et al, created a mouse model in order to develop the antibody pair that could be used in a enzyme-linked immunosorbent assay (ELISA). This is a standard method of measuring most proteins in whole blood, plasma, or sera.
As you can see, the line of best fit indicating optical detection of immunofluorescence and antigen concentration of two different forms of Nattokinase was excellent.
The ELISA was developed using a combination of new mouse anti-nattokinase mAbs used as capture antibodies coated onto 96-well plates, with a peroxidase conjugated antibody used for detection. This ELISA enabled detection of nattokinase at 1 ng/mL.
This means the field of Nattokinase research will advance with pharmacokinetics and potentially target treatment studies to find the ideal dose and blood levels of Nattokinase needed to best manage the bourgeoning numbers of patients with post-acute sequalae after recurrent COVID-19 infection and or ill-advised COVID-19 vaccination.
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Peter A. McCullough, MD, MPH
President, McCullough Foundation
Good news. So glad that researchers continue to study nattokinase and its role in dissolving spike protein. Thank you, Dr. McCullough for sharing your amazing breadth of knowledge about all things COVID. Now, how do I protect myself, a pure blood who has never been jabbed and never had COVID, but now live in a retirement community (a congregate living setting) from all of the other residents who are getting their nth COVID booster and, possibly shedding exosomes of mRNA and spike protein? For many years, long before COVID, I have been taking Tumeric Curcumin 1000 mg twice daily and vitamin D and other supplements. I have also been on daily aspirin 325mg a day for about 8 years.
A related question for me is how long do the anti- clotting effects last after you stop taking it? I give blood several times a year, so it seems logical that I'd want to stop natto some days prior. Maybe the Japanese Red Cross has guidelines for this?